Coding
comDE
Part:BBa_K1351016:Design
Designed by: Judith Heckmann Group: iGEM14_LMU-Munich (2014-10-02)
comDE Quorum Sensing two component system (Freiburg Standard)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1164
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Mutation of two EcoRI and one SpeI recognition sites in comDE
Source
gDNA of Streptococcus pneumoniae R6